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Gram-Weigart Stain - Solution IIB - 2.3% Aqueous Aniline Oil (250mL)

$130.00
Catalog No.
ENG 7163
MFR. No.
7163
MFR. Name
ENG Scientific
Quantity
250mL
Unit of Measure
BO

Aqueous Aniline Oil solution IIB for Gram-Weigart staining, supplied in a bottle for specific staining of bacteria with enhanced contrast and visibility in microbiological samples.

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  • Solution I - Eosin Y, (1%) Aqueous
  • Solution IIA - Crystal Violet
  • Solution IIB - Aniline Oil (2.3%) Aqueous
  • Solution III - Grams Iodine
  • Solution IV - Aniline Oil/Xylene
  • Solution V - Xylene

This stain kit is useful in demonstrating Pneumocystis carinii in smears of sputum, transtracheal aspirates, and bronchial washings, however, lung biopsy specimens obtained by percutaneous needle aspiration provide a better opportunity for diagnosis. Tissue sections and imprints may also be used. Prior to staining, imprints and smears must be air-dried and fixed in methanol.

CONTENTS: Eosin Y, Crystal Violet, Ethanol, Aniline Oil, Iodine, Potassium Iodide, Xylene

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Working Solution: Mix 10 ml of Sol. IIA with 90 ml of Sol. IIB. Stain air-dried smears or imprints in Sol. I for 3 - 5 minutes. Rinse with water. Stain in working solution for 5 - 10 minutes. Rinse in Sol. III for 5 - 10 minutes. Rinse in water and blot dry. Decolorize in Sol. IV and rinse in Sol. V.

RESULTS: Pneumocystis appears blue to deep purple against a pink background.

Safety Data Sheet - Gram-Weigart Stain Kit (Solution IIB)

UNSPSC:
12352142
Condition:
New
Availability:
3-5 Days
Weight:
0.65 Ounces
HazMatClass:
No
WeightUOM:
LB