Ziehl-Neelsen Stain - Solution III - Loeffler's Methylene Blue (2 x 250mL)

• Solution I - Carbol Fuchsin
• Solution II - Acid Alcohol
• Solution III - Loeffler’s Methylene Blue

CONTENTS: Basic Fuchsin, Phenol, Ethanol, HCl, Methylene Blue

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Flood slide with Sol. I and heat until steam rises. Use low or intermittent heat to maintain steam for 3 - 5 minutes. After cooling, wash briefly with water and decolorize with Sol. II. Wash with water and counterstain with Sol. III for 20 - 30 seconds. Rinse with water, allow to dry and examine.

RESULTS: Acid-fast organisms stain red; other organisms and material stain blue.

Safety Data Sheet - Ziehl-Neelsen Stain Kit (Solution III)