Cyclospora Stain Kit - Solution III - Malachite Green (2 x 250mL)

• Solution I - Carbol Fuchsin
• Solution II - Acid Alcohol (10% Sulfuric)
• Solution III - Malachite Green

CONTENTS: Basic Fuchsin, Ethanol, Phenol, Sulfuric Acid, Malachite Green

These solutions are made from certified dyes (when applicable).

FOR IN VITRO DIAGNOSTIC USE ONLY.

RECOMMENDED PROCEDURE: Specimen may be prepared from fresh material or preserved in 10% buffered formalin (Product # 9700). Formalin-preserved Specimens: Mix stool specimen thoroughly and filter an adequate amount through cheesecloth into a centrifuge tube. Centrifuge for 2 minutes and decant supernatant. Smear 1 to 2 drops of specimen on a glass slide (either concentrate or original specimen). Heat fix on slide warmer until dry (about 5 minutes). Fresh Stool Specimens: Prepare smear utilizing the same procedure as for a formalin-fixed specimen. Fix slide in absolute methanol - 30 seconds. Flood slide with Sol. I for 1 minute. (Enhanced staining can be obtained by increasing time or applying heat). Rinse with distilled water and drain. Decolorize with Sol. II for 2 minutes. Rinse with distilled water and drain. Counterstain with Sol. III for 2 minutes. Rinse briefly with distilled water and drain. Dry on slide warmer until dry and mount.

RESULTS: Cyclospora oocysts stain light pink to deep red measuring 8-10 microns.

Safety Data Sheet - Cyclospora Stain Kit (Solution III)